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| Registros recuperados : 7 | |
| Registros recuperados : 7 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
20/11/2007 |
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Data da última atualização: |
24/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
MÉO, S. C.; YAMAZAKI, W.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; LEAL, C. L. V.; GARCIA, J. M. |
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Afiliação: |
SIMONE CRISTINA MEO, CPPSE; W. YAMAZAKI, Unesp/Jaboticabal; C. R. FERREIRA, Unesp/Jaboticabal; F. PERECIN, Unesp/Jaboticabal; N. Z. SARAIVA, Unesp/ Jaboticabal; C. L. V. LEAL, FZEA/USP; J. M. GARCIA, Unesp/Jaboticabal. |
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Título: |
Parthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Zygote, v. 15, p. 295-306, nov. 2007. |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro-matured (IVM) bovine oocytes were activated with single and combined treatments of strontium (S), ionomycin (I) and 6-DMAP (D). Using oocytes IVM for 26 h, we observed that activation altered cell cycle kinetics (faster progression, MIII arrest, or direct transition from MII to pronuclear stage) when compared to in vitro fertilization. The effect of oocyte age on early parthenogenesis was assessed in oocytes IVM for 22, 26 and 30 h. Better results in pronuclear development were obtained in treatments ISD (81.7%) at 22 h; D (66.7%), IS (63.3%), ID (73.3%) and ISD (76.7%) at 26 h; and D (86.7%), IS (85.0%) and ID (78.3%) at 30 h. Higher cleavage occurred on ISD (80.0%) at 22 h; ID (83.3%) and ISD (91.7%) at 26 h; and I (86.7%), IS (90.0%), ID (85.0%) and ISD (95.0%) at 30 h. More blastocysts were achieved in ID (25.0%) and ISD (18.3%) at 22 h; and in ID at 26 h (45.0%) and 30 h (50.0%). We also observed that IS allowed higher haploid (77.4%) embryonic development, whilst ID was better for diploid (89.1%) development. It was concluded that association of S and D without I was not effective for blastocyst development; treatments using S were less influenced by oocyte age, but when S was associated with D here was a detrimental effect on aged oocytes; treatment ISD promoted higher activation and cleavage rates in young oocytes and ID protocol was the best for producing blastocysts. |
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Palavras-Chave: |
Embryonic development; Oocyte activation. |
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Thesaurus NAL: |
cattle; chromatin; microtubules. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02150naa a2200253 a 4500
001 1048149
005 2023-02-24
008 2007 bl uuuu u00u1 u #d
100 1 $aMÉO, S. C.
245 $aParthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments.$h[electronic resource]
260 $c2007
520 $aIn vitro-matured (IVM) bovine oocytes were activated with single and combined treatments of strontium (S), ionomycin (I) and 6-DMAP (D). Using oocytes IVM for 26 h, we observed that activation altered cell cycle kinetics (faster progression, MIII arrest, or direct transition from MII to pronuclear stage) when compared to in vitro fertilization. The effect of oocyte age on early parthenogenesis was assessed in oocytes IVM for 22, 26 and 30 h. Better results in pronuclear development were obtained in treatments ISD (81.7%) at 22 h; D (66.7%), IS (63.3%), ID (73.3%) and ISD (76.7%) at 26 h; and D (86.7%), IS (85.0%) and ID (78.3%) at 30 h. Higher cleavage occurred on ISD (80.0%) at 22 h; ID (83.3%) and ISD (91.7%) at 26 h; and I (86.7%), IS (90.0%), ID (85.0%) and ISD (95.0%) at 30 h. More blastocysts were achieved in ID (25.0%) and ISD (18.3%) at 22 h; and in ID at 26 h (45.0%) and 30 h (50.0%). We also observed that IS allowed higher haploid (77.4%) embryonic development, whilst ID was better for diploid (89.1%) development. It was concluded that association of S and D without I was not effective for blastocyst development; treatments using S were less influenced by oocyte age, but when S was associated with D here was a detrimental effect on aged oocytes; treatment ISD promoted higher activation and cleavage rates in young oocytes and ID protocol was the best for producing blastocysts.
650 $acattle
650 $achromatin
650 $amicrotubules
653 $aEmbryonic development
653 $aOocyte activation
700 1 $aYAMAZAKI, W.
700 1 $aFERREIRA, C. R.
700 1 $aPERECIN, F.
700 1 $aSARAIVA, N. Z.
700 1 $aLEAL, C. L. V.
700 1 $aGARCIA, J. M.
773 $tZygote$gv. 15, p. 295-306, nov. 2007.
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